Illumina NovaSeq X Plus Sequencing System

Illumina NovaSeq X Plus: premium buying summary

The Illumina NovaSeq X Plus is the dual-flow-cell member of the NovaSeq X Series, designed for very high-throughput research sequencing programs. It can run one or two flow cells, and Illumina states that a dual-flow-cell run delivers twice the output listed for a single flow cell. With current 25B flow cells, per-flow-cell 2 × 150 bp output is approximately 8–10.5 Tb, placing the platform in a population-scale sequencing class.

Key Illumina NovaSeq X Plus specification highlights

• Dual-flow-cell architecture with the ability to run one or two flow cells.
• Current 1.5B, 5B, 10B and 25B flow-cell options.
• Up to approximately 8–10.5 Tb per 25B flow cell at 2 × 150 bp; dual-flow-cell runs deliver twice the listed per-flow-cell output.
• Up to approximately 26–35B single reads or 52–70B paired-end reads per 25B flow cell under Illumina’s current reference ranges.
• 86.4 × 93.3 × 158.8 cm instrument size with monitor; dry weight 1171 lb without UPS.
• 200–240 VAC, 50/60 Hz, 15A single-phase and dedicated 10GbE network planning.

Illumina NovaSeq X Plus product overview

NovaSeq X Plus should be bought as a sequencing infrastructure platform, not simply as a faster sequencer. The physical instrument, UPS, data-management connection, reagent logistics, primary/secondary analysis, sample tracking and storage all need to be designed around its throughput. Illumina’s current 25B range can generate several terabases from one flow cell, so downstream informatics and data movement can become the limiting factor if they are not planned at the same time as the instrument.

Illumina NovaSeq X Plus price, cost and quotation considerations

NovaSeq X Plus price comparisons should include the complete installed workflow: instrument, UPS and installation, flow cells, XLEAP-SBS reagents, library preparation, service, local or cloud analysis, 10GbE networking and large-scale data storage. Because the Plus model can operate with two flow cells, utilization rate is central to cost per sample. A laboratory that cannot keep both sides productively loaded may not capture the same economics as a high-volume sequencing center.

Core features and capabilities

• Single- or dual-flow-cell operation.
• High-density 25B flow-cell support in the current NovaSeq X Series portfolio.
• XLEAP-SBS chemistry designed for high-throughput short-read sequencing.
• High-throughput compute and data-management architecture integrated with the instrument workflow.
• Dedicated 10GBASE-T network connection requirement to the data-management system.
• Scales from lower-output flow cells to population-scale 25B workflows without changing instrument family.

Detailed Illumina NovaSeq X Plus technical specifications

Performance and use-case information

The defining NovaSeq X Plus performance characteristic is dual-flow-cell throughput. Illumina publishes output per flow cell, then states that a dual-flow-cell run delivers twice that amount. For 25B at 2 × 150 bp, the listed per-flow-cell range is about 8–10.5 Tb. Actual output is not guaranteed at the top of the range and depends on library type, sample type and run optimization, so capacity planning should use conservative production assumptions.

Compatibility, software, connectivity and site requirements

Site readiness is a major part of compatibility. The instrument needs appropriate floor loading, delivery clearance, electrical service, UPS placement, temperature/humidity control and dedicated 10GbE connectivity. Informatics teams should confirm where primary and secondary analysis data will be stored, whether BaseSpace Sequence Hub or Illumina Connected Analytics is used, and whether local storage/network infrastructure can sustain multi-gigabit transfers from high-output runs.

Applications for Illumina NovaSeq X Plus

• Population-scale whole-genome sequencing
• Large oncology research cohorts
• High-throughput exome sequencing
• Large transcriptomics programs
• Biobank and population genomics
• Large-scale rare-disease research
• Sequencing cores serving many concurrent projects

Illumina NovaSeq X Plus comparison with related models

NovaSeq X Plus versus NovaSeq X is primarily a flow-cell concurrency decision. Both belong to the same NovaSeq X Series and share the same core 25B capability per flow cell, physical installation class and site requirements. NovaSeq X runs one flow cell, while X Plus can run one or two and doubles listed output when both are used. If weekly demand cannot keep two flow cells efficiently utilized, NovaSeq X may be the more rational capacity match.

What is included?

A NovaSeq X Plus purchase should explicitly identify the instrument, monitor/computing components, UPS, installation, accessories pallet contents, training, service plan and any consumables included in the commercial package. Do not assume 25B flow cells, reagent kits, library-preparation kits or long-term data services are bundled unless they appear on the quotation.

Important limitations and buyer considerations

• Very large and heavy floor-standing system; logistics and floor loading must be planned.
• Dual-flow-cell capacity only creates value if sample volume and informatics can keep pace.
• Top-of-range output is not guaranteed and depends on library/run optimization.
• 10GbE and multi-gigabit data movement can require storage/network upgrades.
• The research-use labeling must be respected.
• Service, warranty and installation scope should be confirmed for the destination.

Who should buy this exact model?

The Illumina NovaSeq X Plus is appropriate for high-volume sequencing centers, population-genomics programs and organizations that can use dual-flow-cell capacity consistently. Lower-volume laboratories should compare NovaSeq X or NextSeq 2000 before committing to the larger installed and operating footprint.

Authoritative technical and manufacturer resources

• Official manufacturer product/specification resource
• Official technical/support documentation

For this sequencing platform, confirm the current Illumina flow-cell/software compatibility matrix, site-readiness requirements and service coverage.

Illumina NovaSeq X Plus technical quick answers

What is the Illumina NovaSeq X Plus maximum output?

Illumina lists about 8–10.5 Tb per 25B flow cell at 2 × 150 bp; a dual-flow-cell run delivers twice the listed per-flow-cell output.

How much does the the NovaSeq X Plus system weigh?

Dry weight is 1171 lb without UPS and 1253 lb with UPS; loaded wet weight is listed at 1296 lb.

What are the the NovaSeq X Plus system dimensions?

86.4 × 93.3 × 158.8 cm with monitor.

What is the difference between NovaSeq X Plus and NovaSeq X?

X Plus supports one or two flow cells; NovaSeq X is single-flow-cell.

Detailed FAQs

Does the the NovaSeq X Plus system require 10GbE?

The current specification sheet calls for a dedicated 10GBASE-T connection between the instrument and data-management system.

Can the the NovaSeq X Plus system run only one flow cell?

Yes. Illumina states it can perform single- or dual-flow-cell runs.

Are 25B flow cells always included?

No universal bundle should be assumed. The quotation must state included consumables.

What should a site survey cover?

Delivery route, floor loading, electrical service, UPS, ventilation, temperature/humidity, 10GbE network, storage and service access.

Is the NovaSeq X Plus for clinical diagnosis?

The cited current specification sheet is labeled For Research Use Only and not for use in diagnostic procedures.

Relevant internal links

• Illumina NovaSeq X
• Illumina NextSeq 2000
• Illumina NextSeq 1000

Request a B2B quotation

For a NovaSeq X Plus quotation, provide expected annual throughput, flow-cell plan, analysis/storage architecture, destination and site-readiness status so installation and consumables can be scoped correctly.