Illumina NovaSeq X Sequencing System
$591,000.00
Illumina NovaSeq X — The Illumina NovaSeq X is the single-flow-cell member of the NovaSeq X Series, providing access to current 1.5B, 5B, 10B and 25B flow-cell workflows without the second flow-cell position of the NovaSeq X Plus. A 25B flow cell at 2 × 150 bp is specified at approximately 8–10.5 Tb per flow cell, making NovaSeq X suitable for very large sequencing studies where single-flow-cell capacity is sufficient. This page is structured for buyers comparing Illumina NovaSeq X specifications, price, compatibility and the exact configuration required before a B2B order.
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Illumina NovaSeq X: premium buying summary
The Illumina NovaSeq X is the single-flow-cell member of the NovaSeq X Series, providing access to current 1.5B, 5B, 10B and 25B flow-cell workflows without the second flow-cell position of the NovaSeq X Plus. A 25B flow cell at 2 × 150 bp is specified at approximately 8–10.5 Tb per flow cell, making NovaSeq X suitable for very large sequencing studies where single-flow-cell capacity is sufficient.
Key Illumina NovaSeq X specification highlights
- Single-flow-cell NovaSeq X Series configuration.
- Supports current 1.5B, 5B, 10B and 25B flow-cell classes.
- Up to approximately 8–10.5 Tb at 2 × 150 bp on one 25B flow cell.
- Approximately 26–35B single reads / 52–70B paired-end reads per 25B flow cell under Illumina reference ranges.
- Same 86.4 × 93.3 × 158.8 cm instrument class as the NovaSeq X Series.
- Requires large-scale power, UPS, 10GbE and data-storage planning.
Product overview
NovaSeq X gives laboratories access to the NovaSeq X chemistry and 25B capacity while limiting the system to one flow cell per run. That can be advantageous when a laboratory needs very high output but does not have the sustained sample volume required to use two flow cells efficiently. It also simplifies capacity planning relative to X Plus while maintaining the same broad sequencing applications.
Illumina NovaSeq X price, cost and quotation considerations
When evaluating NovaSeq X cost, compare actual annual sequencing volume against NovaSeq X Plus and NextSeq 2000. The most economical instrument is not automatically the lowest purchase price: utilization, reagent consumption, data infrastructure, service and staffing all affect cost per sample. A single-flow-cell NovaSeq X may be more efficient than X Plus where demand is high but not consistently high enough for dual-flow-cell operation.
Core features and capabilities
- Current 25B high-density flow-cell capability.
- XLEAP-SBS chemistry for high-throughput short-read sequencing.
- Single-flow-cell operation simplifies scheduling for large but discrete projects.
- Dedicated high-throughput compute/data workflow within the NovaSeq X Series architecture.
- 10GbE data-management connection.
- Supports multiple flow-cell sizes so labs do not need to use maximum-output consumables for every project.
Detailed Illumina NovaSeq X technical specifications
| Flow-cell operation | Single flow cell |
|---|---|
| Current flow-cell classes | 1.5B, 5B, 10B, 25B |
| 2 × 150 bp output | ~0.5–0.716 Tb (1.5B); ~1.5–2 Tb (5B); ~3–4 Tb (10B); ~8–10.5 Tb (25B) |
| Reads passing filter, 25B | ~26–35B single reads / ~52–70B paired-end reads |
| Dimensions with monitor | 86.4 × 93.3 × 158.8 cm |
| Dry weight without UPS | 1171 lb |
| Dry weight with UPS | 1253 lb |
| Wet loaded weight | 1296 lb |
| Crated weight | 1591 lb / 722 kg; accessories pallet 525 lb / 238 kg |
| Power | 200–240 VAC, 50/60 Hz, 15A single-phase |
| Network | Dedicated 10GbE / 10GBASE-T RJ-45 |
| Bandwidth guidance | 800 Mbit/s primary-analysis upload; 3.2 Gbit/s primary + secondary analysis upload |
| Operating environment | 15–25°C; 20–65% RH non-condensing; altitude below 2000 m |
| Research-use statement | For Research Use Only; not for use in diagnostic procedures |
Performance and use-case information
One 25B flow cell can produce the same listed per-flow-cell output range as on the X Plus platform. The distinction is concurrency, not a lower-performance flow cell. Buyers should therefore model weekly sample volume, turnaround commitments and queueing. If one 25B run already meets project demand, a second flow-cell position may add cost without enough utilization benefit.
Compatibility, software, connectivity and site requirements
The system requires the same serious site planning as X Plus: delivery path, floor support, 200–240V electrical service, UPS, environmental control, 10GbE and storage capable of ingesting very large datasets. Analysis strategy should be designed before installation so the instrument is not blocked by downstream compute or transfer bottlenecks.
Applications
- Population genomics
- Large whole-genome sequencing projects
- Oncology research
- Large exome studies
- Transcriptomics at scale
- Core-facility high-throughput sequencing
- Large rare-disease and biobank programs
Comparison with closely related models
NovaSeq X versus X Plus is a capacity-utilization decision. Both use the same NovaSeq X Series flow-cell classes and have the same per-flow-cell output table. X Plus can run two flow cells and doubles listed output when both are used; NovaSeq X runs one. Against NextSeq 2000, NovaSeq X moves into a much higher output and infrastructure class, so laboratories should compare annual sample volume and turnaround requirements rather than headline purchase price alone.
What is included?
Confirm the instrument, UPS, installation, compute/data components, accessories, training, service coverage and any starter consumables in writing. Flow cells and reagent kits are recurring consumables and should not be assumed to be bundled.
Important limitations and buyer considerations
- Large physical and data-center-like laboratory footprint compared with NextSeq.
- Single-flow-cell throughput can still exceed a laboratory’s downstream analysis capacity.
- Maximum output range depends on library/sample/run optimization.
- Requires 10GbE and substantial storage planning.
- RUO labeling applies to the cited configuration.
- Service and warranty are configuration/region dependent.
Who should buy this exact model?
The Illumina NovaSeq X is suited to laboratories that need NovaSeq X Series 25B capability but do not require simultaneous dual-flow-cell capacity. It is especially compelling when one high-output flow cell matches the laboratory’s project cadence.
Authoritative technical and manufacturer resources
For this sequencing platform, confirm the current NovaSeq X flow-cell/software requirements, site readiness and Illumina service coverage.
Illumina NovaSeq X technical quick answers
What is the Illumina NovaSeq X output with a 25B flow cell?
About 8–10.5 Tb per flow cell at 2 × 150 bp under Illumina’s published reference range.
How many flow cells can the the NovaSeq X system run?
One flow cell per run.
What are the the NovaSeq X system dimensions?
86.4 × 93.3 × 158.8 cm with monitor.
How is the NovaSeq X system different from X Plus?
The flow-cell capability is the same per flow cell, but X Plus can run two flow cells and NovaSeq X runs one.
Detailed FAQs
Does the the NovaSeq X system support 25B flow cells?
Yes, the current NovaSeq X Series specification includes 25B.
What network connection is required?
The current specification calls for dedicated 10GBASE-T connectivity to the data-management system.
Is NovaSeq X lighter than NovaSeq X Plus?
Illumina publishes a shared NovaSeq X Series instrument specification; confirm the exact purchased configuration during site planning.
What should be confirmed on a quote?
Instrument configuration, installation, UPS, service, training, included accessories/consumables, lead time and destination logistics.
Can it be used for diagnostic procedures?
The cited current specification sheet is For Research Use Only and not for use in diagnostic procedures.
Relevant internal links
Request a B2B quotation
For a NovaSeq X quotation, provide annual sequencing volume, 25B utilization assumptions, storage/network design, destination and site-readiness requirements before final configuration.
