Illumina NextSeq 1000 Sequencing System
$45,000.00
Illumina NextSeq 1000 — The Illumina NextSeq 1000 is a compact short-read sequencing system built around P1 and P2 flow-cell workflows, integrated DRAGEN analysis and the same physical instrument platform as the NextSeq 2000. It is designed for laboratories that need flexible genomics and transcriptomics throughput but do not require the P3/P4 capacity available on the NextSeq 2000. This page is structured for buyers comparing Illumina NextSeq 1000 specifications, price, compatibility and the exact configuration required before a B2B order.
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Illumina NextSeq 1000: premium buying summary
The Illumina NextSeq 1000 is a compact short-read sequencing system built around P1 and P2 flow-cell workflows, integrated DRAGEN analysis and the same physical instrument platform as the NextSeq 2000. It is designed for laboratories that need flexible genomics and transcriptomics throughput but do not require the P3/P4 capacity available on the NextSeq 2000.
Key Illumina NextSeq 1000 specification highlights
- P1 and P2 XLEAP-SBS flow-cell support.
- Up to 240 Gb with a supported P2 2 × 300 bp workflow, or 120 Gb at 2 × 150 bp.
- Up to 400 million single reads / 800 million paired-end reads on P2.
- Integrated DRAGEN Bio-IT FPGA secondary analysis.
- 55 × 65 × 60 cm footprint and 141 kg instrument weight.
- Same core site-planning requirements as NextSeq 2000, but without P3/P4 throughput.
Illumina NextSeq 1000 product overview
NextSeq 1000 should be evaluated as a flexible P1/P2 platform. Illumina’s current XLEAP-SBS specifications show 10 Gb at P1 2 × 50 bp, 30 Gb at P1 2 × 150 bp and 60 Gb at P1 2 × 300 bp. P2 expands this to 40 Gb at 2 × 50 bp, 80 Gb at 2 × 100 bp, 120 Gb at 2 × 150 bp and 240 Gb at 2 × 300 bp. That range can cover many research sequencing workloads without paying for capacity a lab may not use.
Illumina NextSeq 1000 price, cost and quotation considerations
When comparing NextSeq 1000 price or total cost, separate the instrument from the recurring sequencing workflow. Flow cells, reagent kits, library preparation, data storage, service and staff time can dominate lifetime cost. A lower instrument purchase price is only an advantage if P1/P2 throughput fits the laboratory’s expected run volume and turnaround targets.
Core features and capabilities
- Patterned flow-cell technology with fixed cluster locations.
- XLEAP-SBS chemistry on current P1/P2 workflows.
- Integrated DRAGEN FPGA secondary analysis for supported pipelines.
- Lossless genomic compression capability for supported workflows.
- Read-length flexibility including supported 2 × 300 bp P1/P2 runs.
- Compact laboratory footprint relative to high-throughput NovaSeq X Series.
Detailed Illumina NextSeq 1000 technical specifications
| Instrument configuration | Self-contained dry sequencer with integrated DRAGEN Bio-IT FPGA secondary analysis |
|---|---|
| Supported flow cells | P1 and P2 |
| Maximum output | Up to 240 Gb on supported P2 2 × 300 bp; 120 Gb at P2 2 × 150 bp |
| Reads passing filter | Up to 400M single reads / 800M paired-end reads on P2 |
| Read lengths | Supports configurations through 2 × 300 bp on P1/P2 |
| Q30 reference | ≥90% through supported 2 × 150 bp workflows; ≥85% at 2 × 300 bp under Illumina reference conditions |
| Run times | Examples: ~8h P1 2 × 50; ~12h P2 2 × 50; ~17h P1 2 × 150; ~22h P2 2 × 150; ~42h P2 2 × 300 |
| Dimensions | 55 × 65 × 60 cm |
| Weight | 141 kg; crated weight 232 kg |
| Power | 100–240 VAC, 50/60 Hz |
| Operating environment | 15–30°C; 20–80% RH non-condensing; altitude 0–2000 m |
| Network guidance | 200 MB/s internal/BaseSpace upload guidance in the current specification sheet |
| Research-use statement | For Research Use Only; not for use in diagnostic procedures unless separately authorized |
Performance and use-case information
The practical NextSeq 1000 performance question is whether P2 capacity is enough for the laboratory’s recurring workload. At 2 × 150 bp, P2 is specified at 120 Gb and 400M single reads. For longer 2 × 300 bp work, P2 reaches 240 Gb. Laboratories should convert these figures into samples per run using their own required depth, not rely on a generic sample-count promise.
Compatibility, software, connectivity and site requirements
Confirm assay library compatibility, index scheme, cycle count and local analysis/storage workflow. The integrated DRAGEN engine is useful for supported secondary analysis, but not every custom pipeline should be assumed to run on-instrument. The site must also meet electrical, environmental and network requirements, while reagent and flow-cell storage should follow current Illumina instructions.
Applications for Illumina NextSeq 1000
- Whole-exome sequencing
- RNA-seq
- Small-genome sequencing
- Targeted sequencing panels
- Amplicon sequencing
- Single-cell quality-control or smaller single-cell studies
- Research workflows requiring up to 2 × 300 bp
Comparison with closely related models
NextSeq 1000 and NextSeq 2000 share the same physical dimensions, weight and integrated DRAGEN architecture. The dividing line is flow-cell capacity: NextSeq 1000 uses P1/P2, while NextSeq 2000 adds P3/P4 and therefore extends to much higher read counts and up to 540 Gb at 2 × 150 bp. If a lab expects growth beyond P2, buying the 2000 can reduce the risk of an early throughput ceiling.
What is included?
The exact package should be written in the quotation. Confirm the sequencer, installation, accessories, software/service entitlement, starter materials if any, training and whether any reagent kit or flow cell is actually included. Recurring consumables should be budgeted separately.
Important limitations and buyer considerations
- No P3/P4 flow-cell support.
- Maximum throughput may be insufficient for large population-scale programs.
- Instrument weighs 141 kg and requires planned delivery/bench support.
- Run performance depends on library quality and loading.
- Research-use labeling must be respected.
- Service and warranty coverage depends on the purchase/service agreement and region.
Who should buy this exact model?
The Illumina NextSeq 1000 is a good fit for laboratories that need benchtop NGS with P1/P2 flexibility, integrated analysis and moderate throughput. It is less suitable when P3/P4 capacity is already part of the near-term workload forecast.
Authoritative technical and manufacturer resources
For this sequencing system, verify the current Illumina P1/P2 reagent compatibility, software version and destination-specific service coverage.
Illumina NextSeq 1000 technical quick answers
What is the Illumina NextSeq 1000 maximum output?
Up to 240 Gb on a supported P2 2 × 300 bp run; at 2 × 150 bp, P2 output is 120 Gb.
How many reads does the the NextSeq 1000 system produce?
Illumina lists up to 400M single reads or 800M paired-end reads on P2.
What are the the NextSeq 1000 system dimensions?
55 × 65 × 60 cm; weight 141 kg.
Does the the NextSeq 1000 system support P4?
No. P3 and P4 are available on the NextSeq 2000, not the 1000.
Detailed FAQs
Does the the NextSeq 1000 system include DRAGEN?
Yes. Illumina describes integrated DRAGEN Bio-IT FPGA secondary analysis.
Can it run 2 × 300 bp?
Yes, on supported P1/P2 kits; confirm the current reagent and software requirements.
What is the main reason to buy NextSeq 2000 instead?
The NextSeq 2000 adds P3 and P4, which materially raises maximum output and read counts.
Is the the NextSeq 1000 system a diagnostic instrument?
The current specification sheet is labeled For Research Use Only and not for use in diagnostic procedures unless a separate authorized use applies.
What should be on the quotation?
Exact instrument condition/configuration, installation, service, software, included accessories, consumables if any, training and destination terms.
Relevant internal links
Request a B2B quotation
For a NextSeq 1000 quotation, provide assay mix, expected samples per run, read length, destination and service/training requirements so the P1/P2 workflow can be scoped correctly.
